Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins

BG Wilhelm, S Mandad, S Truckenbrodt, K Kröhnert… - Science, 2014 - science.org
BG Wilhelm, S Mandad, S Truckenbrodt, K Kröhnert, C Schäfer, B Rammner, SJ Koo…
Science, 2014science.org
Synaptic vesicle recycling has long served as a model for the general mechanisms of
cellular trafficking. We used an integrative approach, combining quantitative immunoblotting
and mass spectrometry to determine protein numbers; electron microscopy to measure
organelle numbers, sizes, and positions; and super-resolution fluorescence microscopy to
localize the proteins. Using these data, we generated a three-dimensional model of an
“average” synapse, displaying 300,000 proteins in atomic detail. The copy numbers of …
Synaptic vesicle recycling has long served as a model for the general mechanisms of cellular trafficking. We used an integrative approach, combining quantitative immunoblotting and mass spectrometry to determine protein numbers; electron microscopy to measure organelle numbers, sizes, and positions; and super-resolution fluorescence microscopy to localize the proteins. Using these data, we generated a three-dimensional model of an “average” synapse, displaying 300,000 proteins in atomic detail. The copy numbers of proteins involved in the same step of synaptic vesicle recycling correlated closely. In contrast, copy numbers varied over more than three orders of magnitude between steps, from about 150 copies for the endosomal fusion proteins to more than 20,000 for the exocytotic ones.
AAAS