Mycobacterium tuberculosis and Mycobacterium bovis bacille Calmette-Guérin (BCG) alter
the maturation of their phagosomes and reside within a compartment that resists
acidification and fusion with lysosomes. To define the molecular composition of this
compartment, we developed a novel method for obtaining highly purified phagosomes from
BCG-infected human macrophages and analyzed the phagosomes by Western
immunoblotting and mass spectrometry-based proteomics. Our purification procedure …